本书介绍了蛋白质的生物信息学研究方法，特别是蛋白质翻译后修饰（PTMs）和网络。本书分为四部分，内容包括蛋白质序列、结构和功能分析的基本框架和主要资源；蛋白质PTMs、蛋白质-蛋白质相互作用（PPIs）和蛋白质网络的分析方法和资源，包括PPI预测的工具和PPI和PTM网络的构建方法；蛋白质组学中的生物信息学方法，包括基于质谱的蛋白质组学的计算方法和替代拼接亚型的综合分析，用于功能发现。本书采用非常成功的分子生物学方法系列格式编写，各章包括各自主题的介绍、必要的材料和试剂清单、逐步的、易于复制的实验室或计算方案，以及故障排除和避免已知陷阱的提示。

《蛋白质生物信息学》具有前沿性和全面性。从蛋白质修饰和网络到蛋白质组学，对于那些希望了解最先进的生物信息学数据库和工具、新型计算方法以及系统生物学中蛋白质和蛋白质组数据分析的未来趋势的读者来说，这是一本宝贵的资源。本书对从事生物技术和制药行业以及生物和医学科学、计算机科学与工程等各种学术部门的研究人员很有帮助。

This volume introduces bioinformatics research methods for proteins, with special focus on protein post-translational modifications (PTMs) and networks. This book is organized into four parts and covers the basic framework and major resources for analysis of protein sequence, structure, and function; approaches and resources for analysis of protein PTMs, protein-protein interactions (PPIs) and protein networks, including tools for PPI prediction and approaches for the construction of PPI and PTM networks; and bioinformatics approaches in proteomics, including computational methods for mass spectrometry-based proteomics and integrative analysis for alternative splice isoforms, for functional discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory or computational protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Protein Bioinformatics: From Protein Modifications and Networks to Proteomics is a valuable resource for readers who wish to learn about state-of-the-art bioinformatics databases and tools, novel computational methods, and future trends in protein and proteomic data analysis in systems biology. This book is useful to researchers who work in the biotechnology and pharmaceutical industries, and in various academic departments, such as biological and medical sciences and computer sciences and engineering.